Recombinant Human LIMP-2/LIMPII Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031285

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type III multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD) . Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.

Synonyms

85 kDa Lysosomal Membrane Sialoglycoprotein; AMRF; CD36; CD36 Antigen-Like 2; CD36L2; EPM4; HLGP85; LGP85; LIMP II; LIMPII; Lysosome Membrane Protein 2; Lysosome Membrane Protein II; SCARB2; SR-BII; Scavenger Receptor Class B Member 2

UniProt

Q14108

Accession Number

NP_005497.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Arg 27-Thr 432

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind recombinant human RSPO1 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.4 kDa

Observed Molecular Weight

110-115 kDa

Species

Human