Recombinant Human PARP-1 Protein (His Tag)

Specifications

Catalog number

PKSH031294

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poly (ADP-ribose) polymerase 1 (PRAP1), also known as NAD (+) ADP-ribosyltransferase 1 (ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly (ADP-ribosyl) ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly (ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Synonyms

ADPRT; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1

UniProt

P09874

Accession Number

NP_001609.2

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Trp 1014

Applications

ELISA

Field of Research

Cell biology; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.035 μg/mL.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < -20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

114.5 kDa

Observed Molecular Weight

100-110 kDa

Formulation

Supplied as sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP, 2mM EDTA, pH 7.5.

Species

Human