Recombinant Human CLEC1B/CLEC2 Protein (His Tag)

Specifications

Catalog number

PKSH031326-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.

Synonyms

1810061I13Rik; CLEC2; CLEC2B; PRO1384; QDED721

UniProt

Q9P126

Accession Number

NP_057593.3

Expression System

HEK293 Cells

Tag

N-His

Sequence

Gln 58-Pro 229

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 76 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.71 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.7 kDa

Observed Molecular Weight

35-38 kDa

Species

Human