Recombinant Human IL7RA/CD127 Protein (His Tag)

Specifications

Catalog number

PKSH031327-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7RA signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4 (+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T (CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8 (+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers.

Abbreviation

IL7RA/CD127

Synonyms

CDW; IL7R; CD127; CDW127; IL-7R-alpha; IL7RA; ILRA; IL-7R; IL-7RA; IL-7Rα; Interleukin-7 receptor subunit alpha; IL-7Rα CD 127; CD127 antigen; IL 7R; IL 7R alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL7Ralpha; Interleukin 7 receptor; Interleukin 7 receptor alpha chain; Interleukin 7 receptor isoform H5 6

UniProt

P16871

Accession Number

NP_002176.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gly 236

Applications

ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind biotinylated mouse TSLP-his in functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.3 kDa

Observed Molecular Weight

45-50 kDa

Species

Human