Recombinant Human ACO1/irp1 Protein (His Tag)

Specifications

Catalog number

PKSH031334

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aconitase 1 (ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur (Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase.

Synonyms

ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1

UniProt

P21399

Accession Number

P21399

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 1-Lys 889

Field of Research

Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

101 kDa

Observed Molecular Weight

90 kDa

Species

Human