Recombinant Human ACP1/LMW-PTP Protein (GST Tag)

Specifications

Catalog number

PKSH031337

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca (2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.

Synonyms

ACP1; Adipocyte Acid Phosphatase; HAAP; LMW-PTP; LMW-PTPase; Low Molecular Weight Cytosolic Acid Phosphatase; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Red Cell Acid Phosphatase 1

UniProt

P24666

Accession Number

AAI06012.1

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-His 158

Applications

Enzyme

Field of Research

Cell Biology, Cancer

Endotoxin

Please contact us for more information.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP) . The specific activity is > 65, 000 pmol/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.3 kDa

Observed Molecular Weight

40 kDa

Species

Human