Recombinant Human IL10RB/IL10R2 Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031342

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25) . It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

Abbreviation

IL10RB/IL10R2

Synonyms

CRFB; Interleukin-10 receptor subunit; Cytokine receptor class-II member; Cytokine receptor family 2 member; CRF2; IL-10R; CDW210B; CRF2-4; Cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; IL-10R2; Interleukin-10 receptor subunit 2; Interleukin-10 receptor subunit beta (IL10RB) ; IL10RB; CRFB4; D21S58; D21S66; CRF2-4; IL-10Rb; Cd210; CRF2 4; Cytokine receptor class II; Cytokine receptor class II CRF2 4; Cytokine receptor family II member 4; I10R2; IL-10 receptor subunit beta; IL-10R subunit 2; IL-10R subunit beta; IL10R2; IL-10RB; Interleukin 10 receptor beta; Interleukin-10 receptor subunit beta

UniProt

Q08334

Accession Number

NP_000619.3

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Ser 220

Applications

ELISA

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB-Fc with a linear ranger of 3. 125-25 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.7 kDa

Observed Molecular Weight

75-85 kDa

Species

Human