Recombinant Human C1QB/C1qB Protein (His Tag)

Specifications

Catalog number

PKSH031345

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge.

Synonyms

C1QB

UniProt

P02746

Accession Number

NP_000482.3

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Ala 253

Field of Research

Neuroscience; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25 kDa

Observed Molecular Weight

30 kDa

Species

Human