Recombinant Human IL17RA Protein (His Tag)

Specifications

Catalog number

PKSH031368

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-17 receptor (IL-17R) ; also known as Interleukin-17 receptor A (IL-17RA) and CD217 antigen (CD217) ; is a cytokine receptor which binds interleukin 17. IL-17R/IL-17RA (CD217) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17R/IL-17RA (CD217) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor IL-17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors; this receptor likely has a multimeric structure. Defects in IL-17R/IL-17RA (CD217) are the cause of familial candidiasis type 5 (CANDF5) . CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections; selective against Candida. It is characterized by persistent and/or recurrent infections of the skin; nails and mucous membranes caused by organisms of the genus Candida; mainly Candida albicans.

Abbreviation

IL17RA

Synonyms

IMD; MGC; CTLA; CANDF; CDw; IL17RA; CANDF5; CD217; CDw217; IL-17RA; IL17R; IMD51; hIL-17R; CD127; CD217 antigen; CDW127; CTLA8; HIL 17R; hIL17R; I17RA; IL 17 receptor; IL 17 receptor A; IL 17RA; IL17; IL-17 R alpha; IL-17 receptor A; IL17A receptor; IL-17R; IL17R-A; IL17-RA; IL-17R-alpha; IL7RA; ILRA; Interleukin 17 receptor A; Interleukin-17 receptor A; MGC10262; CDw217; Interleukin-17 receptor A

UniProt

Q96F46

Accession Number

NP_055154.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Trp 320

Applications

Cell culture; ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant human IL17A at 2 μg/ml (100 μl/well) can bind biotinylated human IL17RA with a linear range of 1. 28-32 ng/ml. 2. Measured by its ability to inhibit IL-17-induced IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 0.03-0.12 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.0 kDa

Species

Human