Recombinant Human Ephrin-B1/EFNB1 Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031370

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Synonyms

CFND; CFNS; EFB1; EFL-3; EFL3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; EPLG2; Elk-L; Ephrin-B1; LERK-2; LERK2

UniProt

P98172

Accession Number

NP_004420.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Gly 232

Applications

ELISA

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind human EFNB1 Fc chimera with a linear ranger of 1. 56-25 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.2 kDa

Observed Molecular Weight

64&36 kDa

Species

Human