Recombinant Human Hemopexin/HPX Protein (His Tag)

Specifications

Catalog number

PKSH031380

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hemopexin (HPX) is plasma glycoprotein belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin with two four-bladed beta -propeller folds has been found in other proteins including collagenases and provides sites for protein-protein interactions. The liver is the major synthesizing organ. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.

Synonyms

Hemopexin; Hpx; Hpxn

UniProt

P02790

Accession Number

NP_000604.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-His 462

Applications

ELISA

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind protoporphyrin IX (PPPIX) . Recombinant human Hemopexin binds > 10 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.7 kDa

Observed Molecular Weight

70-75 kDa

Species

Human