Recombinant Human PLA2G1B/PLA2 Protein (His Tag)

Specifications

Catalog number

PKSH031384

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to thephospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

Synonyms

Group IB phospholipase A2; PLA2; PLA2A; PLA2G1B; PPLA2; Phospholipase A2

UniProt

P04054

Accession Number

NP_000919.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 148

Applications

Enzyme

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to hydrolyze 1-Hexadecanoyl-2- (1-pyrenedecanoyl) -sn-glycero-3-phosphomethanol. The specific activity is > 2, 500 pmol/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.2 kDa

Observed Molecular Weight

19 kDa

Species

Human