Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)

Specifications

Catalog number

PKSH031390

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Platelet-activating factor acetylhydrolase; also known as 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycero-phosphocholine esterase; Group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAF 2-acylhydrolase; PLA2G7 and PAFAH; is secreted protein which belongs to theAB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes; including cardiovascular disease. PLA2G7 also represents an important; potentially functional candidate in the pathophysiology of coronary artery disease (CAD) . Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency) . It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.

Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase;2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47; Group-VIIA phospholipase A2; LDL-PLA (2) ; LDL-PLA2; LDL-associated phospholipase A2; Lp; LpPLA2; gVIIA-PLA2; lipoprotein-associated phospholipase A2

UniProt

Q13093

Accession Number

Q13093-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 441

Applications

Enzyme

Field of Research

Immunology, Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5’Dithiobis (2-nitrobenzoic acid) (DTNB) . The specific activity is > 5000 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.2 kDa

Observed Molecular Weight

50-55 kDa

Species

Human