Recombinant Human 14-3-3 sigma/YWHAS Protein

Specifications

Catalog number

PKSH031400

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

14-3-3 protein sigma (YWHAS) ; also known as stratifin (SFN) and epithelial cell marker protein 1; is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases; phosphatases; and transmembrane receptors; and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2; and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally; YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Synonyms

14-3-3 Protein Sigma; Epithelial Cell Marker Protein 1; HME1; SFN; Stratifin

UniProt

P31947

Accession Number

NP_006133.1

Expression System

E.coli

Tag

None

Sequence

Met 1-Ser 248

Field of Research

Signal Transduction; Neuroscience; Stem cells

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

28 kDa

Species

Human