Recombinant Human CD10/Neprilysin Protein

Specifications

Catalog number

PKSH031421

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

Abbreviation

CD10/Neprilysin

Synonyms

SCA; Neutral endopeptidase 24.; CALLA; CD10; CMT2T; NEP; SCA43; SFE; MME; Atriopeptidase; Common acute lymphocytic leukemia antigen; Enkephalinase; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; Skin fibroblast elastase; EPN; NEP; gp100; CD10 antigen; CD10) ; DKFZp686O16152; EC 3.4.24.11; enkephalinase) ; Membrane metallo endopeptidase; Membrane metallo endopeptidase (neutral endopeptidase; Membrane metallo endopeptidase variant 1; Membrane metallo endopeptidase variant 2; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; membrane-associated; MGC126681; MGC126707; neprilysin-390; neprilysin-411

UniProt

P08473

Accession Number

NP_000893.2

Expression System

HEK293 Cells

Tag

None

Sequence

Tyr 52-Trp 750

Applications

Enzyme

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH, (R&D Systems, Catalog # ES005) . The specific activity is > 1, 000 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

80.0 kDa

Observed Molecular Weight

90-100 kDa

Species

Human