Recombinant Human PSA/KLK3 Protein (His Tag)

Specifications

Catalog number

PKSH031436

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

KLK3 (Kallikrein 3) ; also known as Prostate Specific Antigen (PSA) ; kallikrein-related peptidase 3; Gamma-seminoprotein; is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Synonyms

APS; Gamma-Seminoprotein; Hk3; KLK2A1; Kallikrein-3; P-30 Antigen; PSA; Prostate-Specific Antigen; Semenogelase; Seminin

UniProt

P07288

Accession Number

P07288

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 261

Applications

Enzyme

Field of Research

Signal Transduction; Tags & Cell Markers; Neuroscience; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA) . The specific activity is > 100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.3 kDa

Observed Molecular Weight

35 kDa

Species

Human