Recombinant Human Kallikrein 11/KLK11 Protein (His Tag)

Specifications

Catalog number

PKSH031437

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kallikrein-related peptidase 11 (KLK11) ; also known as hippostasin; trypsin-like serine protease and PRSS20; is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions; which is implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist; resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1; except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate.

Synonyms

Hippostasin; KLK11; Kallikrein-11; PRSS20; Serine Protease 20; TLSP; Trypsin-Like Protease; hK11

UniProt

Q9UBX7

Accession Number

NP_006844.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 250

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5, 5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K. M. et al. , 1999, J. Biol. Chem. 274: 30468) . The specific activity is > 200 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.0 kDa

Observed Molecular Weight

40 kDa

Species

Human