Recombinant Human AKT1/PKB/PKBα Protein (His Tag)

Specifications

Catalog number

PKSH031439

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

V-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.

Synonyms

AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

UniProt

P31749

Accession Number

NP_001014431.1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Ala 480

Applications

ELISA

Field of Research

Signal Transduction; Neuroscience; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 87 % as determined by reducing SDS-PAGE.

Bioactivity

1. No Kinase Activity 2. Measured by its ability to bind biotinylated human CD136 in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

57 kDa

Species

Human