Recombinant Human LRAP/ERAP2 Protein (His Tag)

Specifications

Catalog number

PKSH031454

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH (X) 18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.

Synonyms

FLJ23633; FLJ23701; FLJ23807; L-RAP; LRAP; LRAP.ERAP2

UniProt

B2R769

Accession Number

NP_071745.1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Ala 56-Thr 960

Applications

Enzyme

Field of Research

Cell biology; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC) . The specific activity is > 50 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

106 kDa

Observed Molecular Weight

115-125 kDa

Species

Human