Recombinant Human CLK3 Protein (GST Tag)

Specifications

Catalog number

PKSH031459

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2 (T) and hCLK3 (T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.

Synonyms

PHCLK3; PHCLK3/152

UniProt

P49761

Accession Number

NP_003983.2

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Sequence

Met 1-Arg 490

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

85.0 kDa

Observed Molecular Weight

70 kDa

Species

Human