Recombinant Human CD86/B7-2 Protein (aa 1-239, His Tag)

Specifications

Catalog number

PKSH031474

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD86; also known as B-lymphocyte activation antigen B7-2 (referred to as B70) ; is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells; and thus induces the signal pathways which regulate T cell activation and tolerance; cytokine production; and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis; allergic pulmonary inflammation; arthritis; and antiviral responses; and thus is regarded as a promising candidate for immune therapy.

Synonyms

Activation B7-2 Antigen; B7-2; B7.2; B70; BU63; CD28LG2; CD86; CTLA-4 Counter-Receptor B7.2; FUN-1; LAB72; T-Lymphocyte Activation Antigen CD86

Accession Number

NP_008820.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-His 239

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human CD86 at 20 μg/ml (100 μl/well) can bind human CD28 with a linear ranger of 32-800 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.2 kDa

Observed Molecular Weight

55-60 kDa

Species

Human