Recombinant Human CD122/IL-2RB Protein

Specifications

Catalog number

PKSH031478

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Abbreviation

CD122/IL-2RB

Synonyms

P70; IL2RB; CD122; IL15RB; P70-75; IL-2Rβ; p75; IL-2RB; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; High affinity IL-2 receptor subunit beta; Interleukin-15 receptor subunit beta; p70-75 (p75) ; IL-2Rβ CD122 antigen; High affinity IL 2 receptor beta subunit; High affinity IL 2 receptor subunit beta; IL2 receptor; Interleukin 2 receptor beta; interleukin 2 receptor subunit beta; P70 75; P7075

UniProt

P14784

Accession Number

NP_000869.1

Expression System

HEK293 Cells

Tag

None

Sequence

Met 1-Asp 239

Applications

Cell culture; ELISA

Field of Research

Signal Transduction; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL. 2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15.The ED50 for this effect is 1-4μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.2 kDa

Observed Molecular Weight

40 kDa

Species

Human