Recombinant Human c-MET/HGFR Protein (His Tag)

Specifications

Catalog number

PKSH031482-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Synonyms

AUTS9; DFNB97; HGFR; RCCP2; c-Met

UniProt

P08581

Accession Number

NP_000236.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Thr 932

Applications

ELISA

Field of Research

Signal Transduction; Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Recombinant Human HGF/Hepatocyte Growth Factor Protein at 2ug/mL (100uL/well) can bind RecombinantHuman c-MET/HGFR Protein (His Tag), the EC50 is 16-48 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

103 kDa

Observed Molecular Weight

45 kDa

Species

Human