Recombinant Human Carbonic Anhydrase 10/CA10 Protein (His Tag)

Specifications

Catalog number

PKSH031485-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

Synonyms

CA-RP X; CA-RPX; CA10; CARP X; CARPX; Carbonic Anhydrase-Related Protein 10; Carbonic Anhydrase-Related Protein X; Cerebral Protein 15; HUCEP-15

UniProt

Q9NS85

Accession Number

NP_001076002.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 300

Applications

Enzyme

Field of Research

Neuroscience; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its esterase activity. The specific activity is > 10 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33 kDa

Observed Molecular Weight

37 kDa

Species

Human