Recombinant Human Apolipoprotein A-I/ApoAI Protein (Fc Tag)

Specifications

Catalog number

PKSH031487-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

Synonyms

APOA1; Apo-AI; ApoA-I; Apolipoprotein A-I; Apolipoprotein A1

UniProt

P02647

Accession Number

CAA26097.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Gln 267

Applications

ELISA

Field of Research

Cardiovascular, Neuroscience, Signal Transduction, Cancer, Kits, Lysates, Other, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1, The EC50 of biotinylated human SCARB1 is 0.37 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

55.0 kDa

Observed Molecular Weight

55 kDa

Species

Human