Recombinant Human ULBP1/N2DL1 Protein (His Tag)

Specifications

Catalog number

PKSH031492

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor; NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins; and have tissue-specific expressions. ULBP1; also known as RAET1I and NKG2DL1; together with at least ULBP 2 and 3; are well-known ligands for NKG2D; and activate multiple signaling pathways in primary NK cells; resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells; B-cells; erythroleukemia cell lines and in a wide range of tissues including heart; brain; lung; liver and bone marrow; as well as some tumor cells. As an unconventional member of the MHC class I family; ULBP1 function in immune responses; especially in cancer and infectious diseases. Unlike other ULBP members; ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor; and thus might escape the immune surveillance. Furthermore; UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.

Synonyms

RAET1I

UniProt

Q9BZM6

Accession Number

NP_079494.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gly 216

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human ULBP1-His at 10 μg/ml (100 μl/well) can bind human NKG2D, The EC50 of human NKG2D is 0.39-0.91 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.8 kDa

Observed Molecular Weight

28-32 kDa

Species

Human