Recombinant Human HtrA2/Omi Protein (His Tag)

Specifications

Catalog number

PKSH031504

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine protease HTRA2, also known as high temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein which belongs to thepeptidase S1B family. HTRA2 contains onePDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa.

Synonyms

HTRA2; High temperature requirement protein A2; HtrA2; OMI; Omi stress-regulated endoprotease; PRSS25; Serine protease 25; Serine protease HTRA2; Serine proteinase OMI; mitochondrial

UniProt

O43464

Accession Number

O43464-1

Expression System

E.coli

Tag

C-His

Sequence

Ala 134-Glu 458

Applications

Enzyme

Field of Research

Neuroscience, Cell Biology, Cancer, Metabolism

Endotoxin

Please contact us for more information.

Purity

> 87 % as determined by reducing SDS-PAGE.

Bioactivity

Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905) . Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in > 95% cleavage of β-casein, as revealed by SDS-PAGE.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.5 kDa

Observed Molecular Weight

36.5 kDa

Species

Human