Recombinant Human IL1F6/IL36A Protein (His Tag)

Specifications

Catalog number

PKSH031509

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 family member 6 (IL-1F6) ; also known as interleukin 36; alpha (IL36A) ; is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8; in addition to IL-1F9; activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta; suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line; NCI/ADR-RES; which naturally expresses IL-1Rrp2; all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6; IL-1F8; and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6; IL-1F8; and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

Abbreviation

IL1F6/IL36A

Synonyms

FIL; IL1F; Interleukin-1 Family Member; IL-1F; IL36A; FIL1; FIL1 (EPSILON) ; FIL1E; IL-1F6; IL1 (EPSILON) ; IL1F6; FIL1 Epsilon; IL-1 Epsilon; Interleukin-1 Epsilon; Interleukin-1 Family Member 6; Interleukin-36 Alpha; IL1E; IL 1 epsilon; IL 1F6; IL 1H1; IL1RP2; Interleukin 1 epsilon; Interleukin 1 family member 6; Interleukin 1 family member 6 (epsilon) ; Interleukin 36 alpha; MGC129552; MGC129553; MGC151479; MGC151481; OTTMUSP00000012798; RP23-176J12.4

UniProt

Q9UHA7

Accession Number

Q9UHA7

Expression System

E.coli

Tag

N-His

Sequence

Lys 6-Phe158

Field of Research

Immunology

Endotoxin

Please contact us for more information.

Purity

> 99 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.2 kDa

Observed Molecular Weight

20 kDa

Species

Human