Recombinant Human XIAP/BIRC4 Protein (AVI Tag)

Specifications

Catalog number

PKSH031510

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 ubiquitin-protein ligase XIAP / BIRC4, also known as inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP / BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP / BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP / BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP / BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) . XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV) . Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

Synonyms

API3; BIRC4; IAP-3; ILP1; MIHA; XLP2; hIAP-3; hIAP3

UniProt

P98170

Accession Number

NP_001158.2

Expression System

E.coli

Tag

C-Avi

Sequence

Leu 121-Thr 356

Applications

ELISA

Field of Research

Cell biology; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 75 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.1 kDa

Observed Molecular Weight

29.1 kDa

Species

Human