Recombinant Human TCN2 Protein (His Tag)

Specifications

Catalog number

PKSH031522

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

Synonyms

D22S676; D22S750; TC-2; TC2; TCII; Transcobalamin-II

UniProt

P20062

Accession Number

NP_000346.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Trp 427

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His. The EC50 of biotinylated mouse CD320-His is 18-42 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.7 kDa

Observed Molecular Weight

43 kDa

Species

Human