Recombinant Human PDGFRa/CD140a Protein (Fc Tag)

Specifications

Catalog number

PKSH031529

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b) ; are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation; and in addition; particularly important for kidney development. Additionally; PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC) . PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.

Synonyms

Alpha platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140A; PDGFR-2; PDGFR-alpha; PDGFR2; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor recepto; Platelet-derived growth factor receptor alpha; RHEPDGFRA

UniProt

P16234

Accession Number

NP_006197.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Glu 524

Applications

ELISA

Field of Research

Signal Transduction; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to bind human PDGF-C in a functional ELISA. 2. Measured by its ability to bind mouse PDGF-C in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

82.9 kDa

Observed Molecular Weight

120-130 kDa

Species

Human