Recombinant Human Kininogen 1/KNG1 Protein (His Tag)

Specifications

Catalog number

PKSH031545

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kininogen-1, also known as high molecular weight kininogen, williams-Fitzgerald-Flaujeac factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, KNG1 and BDK, is a secreted protein which contains threecystatin domains. Kininogen-1 / KNG1 is a protein from the blood coagulation system as well as the kinin-kallikrein system. It is a protein that adsorbs to the surface of biomaterials that come in contact with blood. Kininogen-1 / KNG1 circulates throughout the blood and quickly adsorbs to the material surfaces. Kininogen-1 / KNG1 is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. Kininogen-1 / KNG1 is one of thekininogens, a class of proteins. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed. When the clinical data were combined, it turned out that all patients, in fact, had a deficiency of the same protein. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

Synonyms

BDK; Fitzgerald Factor; HMWK; High Molecular Weight Kininogen; KNG; KNG1; Kininogen-1; Williams-Fitzgerald-Flaujeac Factor; lpha-2-Thiol Proteinase Inhibitor

UniProt

P01042

Accession Number

NP_001095886.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln 19-Ser 644

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC, R&D Systems, Catalog # ES009.The IC50 value is < 7 nM.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.3 kDa

Species

Human