Recombinant Human IL3RA/CD123 Protein (His Tag)

Specifications

Catalog number

PKSH031551-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

Abbreviation

IL3RA/CD123

Synonyms

IL3RA; CD123; IL3R; IL3RAY; IL3RX; IL3RY; hIL-3Ra; IL-3 Receptor alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R subunitalpha; IL-3RA; IL-3R-alpha; IL-3Receptoralpha; IL-3Rα; IL-4Receptoralpha; IL-5Receptoralpha; IL-6Receptoralpha; IL-7Receptoralpha; Interleukin-3 receptor subunit alpha; IL-3R&alpha

UniProt

P26951

Accession Number

NP_002174.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Arg 305

Applications

ELISA

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind biotinylated human IL3RA with a linear ranger of 0.32-1. 6 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.5 kDa

Observed Molecular Weight

50-60 kDa

Species

Human