Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)

Specifications

Catalog number

PKSH031565

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase A1 (CPA1) is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Synonyms

CPA; CPA1; Carboxypeptidase A1

UniProt

P15085

Accession Number

NP_001859.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Tyr 419

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB) . The specific activity is > 3, 500 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.0 kDa

Observed Molecular Weight

43 kDa

Species

Human