Recombinant Human IL27Ra/TCCR/WSX1 Protein (Fc Tag)

Specifications

Catalog number

PKSH031571

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the β2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4 (+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4 (+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.

Abbreviation

IL27Ra/TCCR/WSX1

Synonyms

CRL; WSX; UNQ; PRO; zcytor; IL27RA; CRL1; IL-27RA; IL27R; TCCR; WSX1; zcytor1; UNQ296; PRO336

UniProt

Q6UWB1

Accession Number

NP_004834.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met 1-Arg 514

Applications

ELISA

Field of Research

Signal Transduction; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human IL27 at 20 μg/ml (100 μl/well) can bind human IL27Ra with a linear ranger of 0.064-1. 6 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

79.0 kDa

Observed Molecular Weight

105-115 kDa

Species

Human