Recombinant Human Carbonic Anhydrase II/CA2 Protein (His Tag)

Specifications

Catalog number

PKSH031582

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Synonyms

CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282

UniProt

P00918

Accession Number

NP_000058.1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Lys 260

Applications

Enzyme

Field of Research

Cell biology; Cardiovascular

Endotoxin

Please contact us for more information.

Purity

> 96 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its esterase activity. The specific activity is > 150 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.0 kDa

Observed Molecular Weight

30 kDa

Species

Human