Recombinant Human MMP-3 Protein

Specifications

Catalog number

PKSH031588

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs) . MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.

Synonyms

CHDS6; MMP-3; MMP3; Matrix metalloproteinase-3; SL-1; STMY; STMY1; STR1; Stromelysin-1; Transin-1

UniProt

P08254

Accession Number

AAA36321.1

Expression System

E.coli

Tag

None

Sequence

Tyr 18-Thr 272

Applications

Enzyme

Field of Research

Cardiovascular, Signal Transduction, Cancer, Cell Biology, Kits, Lysates, Other

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K (Dnp) -NH2, AnaSpec, Catalog # 27114.The specific activity is > 300 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

34 kDa

Species

Human