Recombinant Human Carbonic Anhydrase 14/CA14 Protein (HEK293 Cells, His Tag)

Specifications

Catalog number

PKSH031597

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Synonyms

CA-XIV; CA14; Carbonate Dehydratase XIV; Carbonic Anhydrase 14; Carbonic Anhydrase XIV; UNQ690/PRO1335

UniProt

A8K3J4

Accession Number

NP_036245.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Met 290

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.3 kDa

Observed Molecular Weight

45-48 kDa

Species

Human