Recombinant Human BCL2L1/Bcl-XL Protein (His Tag)

Specifications

Catalog number

PKSH031599

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α -helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices α l and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Synonyms

Apoptosis Regulator Bcl-X; BCL-XL/S; BCL2L; BCLX; BCLXL; BCLXS; Bcl-2-Like Protein 1; Bcl-X; Bcl2-L-1; PPP1R52; bcl-xL; bcl-xS

UniProt

A0A0S2Z3C5

Accession Number

NP_612815.1

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Arg 212

Applications

ELISA

Field of Research

Signal Transduction; Cell biology; Cancer; metabolism

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL. 2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.2 kDa

Observed Molecular Weight

32 kDa

Species

Human