Recombinant Human FLT-3/FLK-2 Protein (His Tag)

Specifications

Catalog number

PKSH031603-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeIII for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.

Synonyms

CD135; FL Cytokine Receptor; FLK-2; FLK2; FLT-3; FLT3; Fetal Liver Kinase-2; Fms-Like Tyrosine Kinase 3; Receptor-Type Tyrosine-Protein Kinase FLT3; STK-1; STK1; Stem Cell Tyrosine Kinase 1

UniProt

P36888

Accession Number

NP_004110.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 541

Applications

ELISA

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to bind biotinylated human FLT3L-His in a functional ELISA.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

59.7 kDa

Observed Molecular Weight

85-95 kDa

Species

Human