Recombinant Human Alkaline Phosphatase/ALPL Protein (His Tag)

Specifications

Catalog number

PKSH031604

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes) . Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP) . The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.

Synonyms

ALPL; AP-TNAP; Alkaline Phosphatase; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; HOPS; TNAP; TNSALP; Tissue-Nonspecific Isozyme

UniProt

P05186

Accession Number

NP_000469.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 502

Applications

Enzyme

Field of Research

Tags & Cell Markers; Cell biology; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) . The specific activity is > 50, 000 pmoles/min/μg.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

55.0 kDa

Observed Molecular Weight

65 kDa

Species

Human