Recombinant Human Kallikrein 1/KLK1 Protein (His Tag)

Specifications

Catalog number

PKSH031628-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human tissue kallikrein (KLK1) is a serine protease, component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its antiinflammatory, antiapoptotic, antifibrotic and antioxidative actions.Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension.

Synonyms

KLK1; Kallikrein-1; Kidney/Pancreas/Salivary Gland Kallikrein; Tissue Kallikrein

UniProt

P06870

Accession Number

NP_002248.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Ser 262

Applications

Enzyme

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) . The specific activity is > 1, 500 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

50 kDa

Species

Human