Recombinant Human IL6RA/CD126 Protein (His Tag)

Specifications

Catalog number

PKSH031632-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Abbreviation

IL6RA/CD126

Synonyms

Membrane glycoprotein; CD126; gp80; IL6Q; IL-6R; IL-6R 1; IL-6R subunit alpha; IL-6R-1; IL6RA; IL-6RA; IL-6R-Alpha; IL6RQ; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; IL-6Rα alpha; CD 126; CD126 antigen; IL 6R; IL 6R 1; IL 6R alpha; IL-6 receptor alpha chain; IL-6 receptor subunit alpha; IL-6R 1 Membrane glycoprotein 80 (gp80) ; Interleukin 6 receptor; MGC30256

UniProt

P08887

Accession Number

NP_000556.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro 365

Applications

Cell culture; ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1. 25-20.0 ng/ml. 2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40 kDa

Species

Human