Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)

Specifications

Catalog number

PKSH031645-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sonic HedgeHog; also known as sonic hedgehog protein; belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine; liver; lung; and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube; the anterior-posterior limb axis; and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites; and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor; which functions in association with smoothened; to activate the transcription of target genes. In the absence of sonic HedgeHog; patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor; the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5; triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.

Synonyms

HHG-1; SHH; Sonic Hedgehog Protein

UniProt

Q15465

Accession Number

Q15465

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gly 197

Applications

Cell culture

Field of Research

Cancer; Developmental Biology; epigenetics and nuclear signal; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21 kDa

Observed Molecular Weight

24 kDa

Species

Human