Recombinant Human ICOS/AILIM Protein (His & Fc Tag)

Specifications

Catalog number

PKSH031664

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T− B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.

Synonyms

AILIM; CD278; CVID1; ICOS; Inducible T-cell costimulator; activation-inducible lymphocyte immunomediatory molecule

UniProt

Q53QY6

Accession Number

NP_036224.1

Expression System

HEK293 Cells

Tag

C-His-Fc

Sequence

Met 1-Phe 141

Applications

ELISA

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1. 6-200 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.6 kDa

Observed Molecular Weight

50 kDa

Species

Human