Recombinant Human SLPI Protein (aa 1-132, His Tag)

Specifications

Catalog number

PKSH031665

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secretory leukoprotease inhibitor (SLPI) ; also called antileukoprotease (ALP) ; is a 12-kDa; nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells; including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites; and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract; possessing serine protease inhibitor activity; antibacterial activity; and anti-inflammatory/immunomodulatory activity.

Synonyms

ALK1; ALP; BLPI; HUSI; HUSI-I; MPI; WAP4; WFDC4

UniProt

P03973

Accession Number

P03973

Expression System

Baculovirus-Insect Cells

Tag

C-His

Sequence

Met 1-Ala132

Applications

Enzyme

Field of Research

Cell biology; Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Catalog # ES002) . The IC50 value is < 1 Nm.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.1 kDa

Observed Molecular Weight

15 kDa

Species

Human