Recombinant Human Galectin-8/LGALS8 Protein (GST Tag)

Specifications

Catalog number

PKSH031698

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Synonyms

Gal-8; Galectin-8; LGALS8; PCTA-1; Po66 Carbohydrate-Binding Protein; Po66-CBP; Prostate Carcinoma Tumor Antigen 1

UniProt

O00214

Accession Number

AAF19370.1

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Trp 317

Applications

Cell culture

Field of Research

Cancer

Endotoxin

Please contact us for more information.

Purity

> 76 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.2-1 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

62.7 kDa

Observed Molecular Weight

60 kDa

Species

Human