Recombinant Human Galectin-3/LGALS3 Protein, Low Endotoxin

Specifications

Catalog number

PKSH031707-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leukotriene A-4 hydrolase; also known as LTA-4 hydrolase; Leukotriene A (4) hydrolase; LTA4H and LTA4; is cytoplasm protein which belongs to thepeptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4) . This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs; arachidonic acid was converted into the unstable intermediate epoxide LTA4; which may in turn be conjugated with glutathione to form the spasmogenic LTC4; or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H) . LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium; and also modulates immune responses. As a bifunctional zinc metalloenzyme; LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

Abbreviation

Galectin-3; LGALS3

Synonyms

35 kDa Lectin; CBP35; Carbohydrate-Binding Protein 35; GAL3; GALBP; GALIG; Gal-3; Galactose-Specific Lectin 3; Galactoside-Binding Protein; Galectin-3; IgE-Binding Protein; L-31; L31; LGALS2; LGALS3; Laminin-Binding Protein; Lectin L-29; MAC2; Mac-2 Antigen; P35

UniProt

P17931

Accession Number

P17931

Expression System

E.coli

Tag

N-His

Sequence

Ala 2-Ile 250

Applications

Cell culture

Field of Research

Cell biology; Neuroscience

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to chemoattract human PBMC using a concentration range of 2.5-25 μg/mL. Note: Results may vary from different PBMC donors.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27 kDa

Observed Molecular Weight

32 kDa

Species

Human