Recombinant Human Pleiotrophin/PTN/HB-GAM Protein

Specifications

Catalog number

PKSH031708-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

Synonyms

HARP; HBGF8; HBNF; NEGF1

UniProt

P21246

Accession Number

NP_002816.1

Expression System

Baculovirus-Insect Cells

Tag

None

Sequence

Met 1-Asp168

Applications

ELISA

Field of Research

Signal Transduction; Neuroscience; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized human PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.3 kDa

Observed Molecular Weight

19 kDa

Species

Human