Recombinant Human OMGP/OMG Protein (aa 1-420, His Tag)

Specifications

Catalog number

PKSH031717

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Oligodendrocyte-myelin glycoprotein; also known as OMG and OMGP; is a cell membrane protein which contains eightLRR (leucine-rich) repeats. OMG / OMGP is a glycosylphosphatidylinositol-anchored protein expressed by neurons and oligodendrocytes in the central nervous system (CNS) . OMG / OMGP is a cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. OMG / OMGP play roles in both the developing and adult central nervous system. OMG / OMGP participats in growth cone collapse and inhibition of neurite outgrowth through its interaction with NgR; the receptor for Nogo. This function requires its leucine-rich repeat domain; a highly conserved region in OMgp during mammal evolution. OMG / OMGP leucine-rich repeat domain is also implicated in the inhibition of cell proliferation. OMG / OMGP may also be involved in the formation and maintenance of myelin sheaths. Cell proliferation; neuronal sprouting and myelination are crucial processes involved in brain development and regeneration after injury.

Synonyms

OMGP

UniProt

P23515

Accession Number

P23515-1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Asn 420

Applications

Cell culture

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Immobilized OMG (0.8 μg/ml, 100 μl/well) will mediate > 20% C6 cell adhesion.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.0 kDa

Observed Molecular Weight

120-130 kDa

Species

Human